|
|
|
| Registros recuperados: 40 | |
|
|
| Silva, Flavia; Nicoli, Jacques R.; Zambonino, Jose-luis; Le Gall, Marie-madeleine; Kaushik, Sadasivam; Gatesoupe, François-joel. |
| The sustainable composition of diets of high nutritional value is of the utmost importance for intensive aquaculture. Digestion and absorption of nutrients depends on the activity of the digestive enzymes, in particular those located in the brush border membrane of enterocytes, which are responsible for the final stages of breaking down and absorption of nutrients. In the present study, the substitution of fish meal by lupin or rapeseed meal in the diet was evaluated on gilthead sea bream (Sparus aurata) and goldfish (Carassius auratus). The objectives were to compare the activities of intestinal brush border enzymes in both species fed the control and experimental diets. When gilthead sea bream were fed the vegetable diets, significantly lower activities... |
| Tipo: Text |
Palavras-chave: Sparus aurata; Carassius auratus; Fish meal replacement; Brush border membrane; Digestive enzymes; Alkaline phosphatase; Aminopeptidase N; Maltase; Glutamyl transpeptidase. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00009/11975/8679.pdf |
| |
|
| |
|
| |
|
|
| Panserat, Stéphane; Blin, C; Medale, Francoise; Plagnes Juan, E; Breque, Jeanine; Krishnamoorthy, J; Kaushik, Sadasivam. |
| The enzyme glucokinase (GK) (EC 2.7.1.1) plays an important role in the control of glucose homeostasis. Qualitative and/or quantitative variations in GK enzyme have been postulated by previous studies to explain why dietary carbohydrate utilisation is lower in gilthead seabream (Sparus aurata) and rainbow trout (Oncorhynchus mykiss) than in common carp (Cyprinus carpio). In this study, we report the isolation and characterisation of a full-length cDNA coding for GK in these teleosts. Amino acid sequences derived from these cDNA clones are highly similar to other vertebrate GKs. These findings, including a detailed phylogenetic analysis, reveal that GK gene highly homologous to mammalian GK exists in these fish species with similar tissue specific... |
| Tipo: Text |
Palavras-chave: Glucose phosphorylation; Dietary carbohydrate; Fish nutrition. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-669.pdf |
| |
|
|
| Beltran, M.; Fernandez-borras, J.; Medale, Francoise; Perez-sanchez, J.; Kaushik, Sadasivam; Blasco, J.. |
| For developing efficient diets, two sets of experiments examined whether the use and allocation of dietary protein can be traced by labelling with stable isotopes (N-15 and C-13) in two culture fish (Oncorhynchus mykiss and Sparus aurata). In the first experiment, natural abundance and tissue distribution of these isotopes were determined, by measuring the delta C-13 and delta N-15 values by isotopic ratio mass spectrometry, in fingerlings (14-17 g) adapted to diets differing in the percentage of fish meal replacement by plant protein sources. For both species, delta N-15 and delta C-13 were greater in tissues with higher protein and lower lipid content. Delta N-15 of diets and tissues decreased as replacement increased, suggesting delta N-15 can be used... |
| Tipo: Text |
Palavras-chave: Sea bream; Rainbow trout; Protein; Diet; Delta N 15; Delta C 13. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6966.pdf |
| |
|
|
| Kaushik, Sadasivam. |
| The general principles behind the bioenergetic approach for predicting growth, as well as for decreasing feed and nutrient losses, have been set forth for salmonids. Given the diversification of fish farming activities around the world and the ever increasing concern for water quality management, it becomes essential to verify whether an approach developed for salmonids is applicable to other species. Given this general background, an attempt is made here to check the theoretical assumptions and technical considerations behind the bioenergetic principles developed for rainbow trout with other freshwater or marine species. From a conceptual point of view, recent literature data do indicate that as far as nitrogen or energy balance is concerned, the general... |
| Tipo: Text |
Palavras-chave: Nutrition; Croissance; Poissons d'eau douce; Poissons de mer; Nutrition; Growth; Freshwater fish; Marine fish. |
| Ano: 1998 |
URL: https://archimer.ifremer.fr/doc/1998/publication-869.pdf |
| |
|
|
| Panserat, Stéphane; Plagnes Juan, E; Kaushik, Sadasivam. |
| Rainbow trout (Oncorhynchus mykiss) are known to use dietary carbohydrates poorly. One of the hypotheses to explain the poor utilisation of dietary glucose by these fish is a dysfunction in nutritional regulation of hepatic glucose metabolism. In this study, we obtained partial clones of rainbow trout cDNAs coding for a glucose transporter (Glut2), and for the enzymes 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (6PF-2K/F-2,6BPase), fructose-1,6-bisphosphatase (FBPase) and pyruvate kinase (PI(), Their deduced amino acid sequences were highly similar to those of mammals (up to 80% similarity), In a study of nutritional regulation, the Glut2 gene was highly expressed in the liver irrespective of the nutritional status of the trout, in agreement with... |
| Tipo: Text |
Palavras-chave: Oncorhynchus mykiss; Trout; Bifunctional enzyme; Pyruvate kinase; Fructose bisphosphatase; Glucose transporter; Glucose metabolism; Liver metabolism. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-415.pdf |
| |
|
|
| Medale, Francoise; Kaushik, Sadasivam. |
| Fish require diets containing 30 to 55% of crude protein and an amino acid supply precisely adapted to meeting the needs for optimal growth. Fish meal is an ideal protein source for aquafeed because it has a high protein level (65% to 72%) with an ideal proportion of all ten indispensable amino acids that meet the requirements of all fish species. However, finding alternatives to fish meal has become an absolute necessity in order to reduce the reliance of aquaculture on marine ingredients. Animal by-product meals (meat meal, bone meal, blood meal, poultry by-product meal, hydrolysed feather meal) are used as alternative protein sources in aquafeed in extra-European countries. Since the use of animal protein in aquafeed is forbidden in Europe (except non... |
| Tipo: Text |
Palavras-chave: Plant proteins; Fish meal; Fish feeding; Antinutritionnal factors; Amino acids. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6556.pdf |
| |
|
|
| Company, Rubén; Calduch Giner, Josep; Perez Sanchez, Jaume; Kaushik, Sadasivam. |
| The common dentex (Dentex dentex) is a sparid fish which is considered a suitable candidate for Mediterranean aquaculture. A seven-weeks feeding trial was conducted over the summer period with common dentex, sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax). All three species were fed to visual satiety with two practical diets with varying crude protein and crude fat levels (55 % protein, 9 % lipids; 46 % protein, 17 % lipids). The initial body weights were 8.2, 11.6 and 17.3 g for common dentex, sea bass and sea bream, respectively. In all cases, specific growth rates were not significantly affected by dietary treatment, but they varied among species (sea bass 1.7-1.8 %; sea bream 2.1-2.3 %; common dentex 3.1-3.2 %). When comparisons between... |
| Tipo: Text |
Palavras-chave: Sparus aurata; Dicentrarchus labrax; Dentex dentex; Growth hormone; Energy availability; Protein retention; Growth performance; Sparus aurata; Dentex dentex I Dicentrarchus labrax; Hormone de croissance; Energie digestible; Rétention protéinique; Croissance. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/publication-863.pdf |
| |
|
| |
|
|
| Ducasse Cabanot, S; Zambonino, Jose-luis; Richard, N; Medale, Francoise; Corraze, Genevieve; Mambrini, M; Robin, Jean; Cahu, Chantal; Kaushik, Sadasivam; Panserat, S. |
| For sustainable aquaculture, the removal of marine resource ingredients in fish diets is an important objective. While most studies focus on the replacement of fish oil by vegetable oil, little is known on the nutritional effects of presence (which corresponds to the control diet) or absence of dietary fish oil. We studied fatty acid composition of brush-border membranes and digestive enzyme activities of the intestine and measured the expression and activities of several enzymes involved in the hepatic intermediary metabolism of rainbow trout (Oncorhynchus mykiss) fed for 7 weeks with or without fish oil. The diets were pair-fed to ensure that fish fed either diet had comparable carbohydrate and protein intakes. Absence of fish oil significantly reduced... |
| Tipo: Text |
Palavras-chave: Rainbow trout; Metabolism; Fish oil; Digestion; Aquaculture. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3506.pdf |
| |
|
|
| Boujard, Thierry; Gelineau, Anne; Coves, Denis; Corraze, Genevieve; Dutto, Gilbert; Gasset, Eric; Kaushik, Sadasivam. |
| Three practical isoproteic (54% protein) diets were formulated to contain graded levels of crude fat (diet L: 10%, diet M: 20% and diet H: 30%). Each diet was assigned unrestrictedly to three and restrictedly to two replicate groups of fish (IBW 243 g). In unrestricted groups, increasing the dietary lipid level led to a significant decrease in voluntary feed intake without affecting growth rate. In the feed-restricted groups, daily growth rates increased with increasing dietary fat levels. There was a significant and inverse effect of the dietary fat content on whole body moisture and fat levels, with highest lipid (ca. 20%) and lowest moisture (ca. 58%) contents in sea bass fed diet containing the highest lipid level; muscle lipid concentration was... |
| Tipo: Text |
Palavras-chave: Phosphorus excretion; Feed intake; Lipid; Digestible energy; European sea bass; Dicentrarchus labrax. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-392.pdf |
| |
|
|
| Gomez Requeni, P; Calduch Giner, J; De Celis, S; Medale, Francoise; Kaushik, Sadasivam; Perez Sanchez, J. |
| The activity of the somatotropic axis was analysed in juvenile rainbow trout (Oncorhynchus mykiss) fed either a fishmeal-based diet (FM) or graded levels of plant proteins to replace 50 % (PP50 diet), 75 % (PP75 diet) or 100 % (PP100 diet) of the fishmeal protein. For this purpose, partial cloning and sequencing of the gene encoding rainbow trout growth hormone receptor (GHR) was first accomplished by RT-PCR, using degenerate primers based on the sequences of non-salmonid fish GHR. Growth rates and energy retention were lowered by the PP75 and PP100 diets and a concurrent and progressive increase in plasma levels of growth hormone (GH) was found. However, no changes in hepatic GH binding and total plasma insulin-like growth factor (IGF)-I levels were... |
| Tipo: Text |
Palavras-chave: Insulin like growth factor; Growth hormone receptor; Growth hormone; Plant proteins; Rainbow trout. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-3620.pdf |
| |
|
|
| Oliva Teles, A; Guedes, M; Vachot, Christianne; Kaushik, Sadasivam. |
| The aim of this trial was to evaluate the effect of dietary nucleic acids on ureagenesis and nitrogen balance in gilthead sea bream juveniles. For that purpose, 5 isonitrogenous (7.25% N) diets were formulated based on fish meal as protein source (FM-control diet) and to partially replace the fish meal nitrogen with two levels of nucleic acids (diets RNA1 and RNA2) or brewers yeast (diets BY1 and BY2). Each diet was fed for 10 weeks, to apparent visual satiety, to triplicate groups of 50 fish each with an average body weight of 12.7 g. Inclusion of nucleic acid N either as brewers yeast or RNA led to significant improvement of feed intake and growth, except for fish fed diet RNA2. There were no differences in final weight between groups fed the yeast-... |
| Tipo: Text |
Palavras-chave: Sea bream; N accretion; Ureagenesis; Brewers yeast; RNA. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-6681.pdf |
| |
|
|
| Gatesoupe, Joel; Huelvan, Christine; Le Bayon, Nicolas; Severe, Armelle; Aasen, Inga Marie; Degnes, Kristin F.; Mazurais, David; Panserat, Stephane; Zambonino, Jose-luis; Kaushik, Sadasivam. |
| The aim of this work was to investigate the catabolic process of three kinds of dietary carbohydrates in the gut of sea bass juveniles, with the possible contribution of the intestinal microbiota to the nutrition of the host, and the subsequent effects on intermediary metabolism. A first diet contained waxy maize (99% amylopectin), a highly digestible form of starch. A second diet was less quickly digestible due to its high amylose content of resistant starch. Two other diets contained fibre instead of starch, either only cellulose as control, or also other non-starch polysaccharides brought by lupin meal. The effect of the diets on the host confirmed previous results, with the stimulation of glucose storage in the liver in sea bass fed the starchy diets,... |
| Tipo: Text |
Palavras-chave: Digestive enzymes; Glucose metabolism; Intestinal microbiota; Viscerosomatic index; Gene expression; Metabolite analyses; Vegetable feedstuffs. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00166/27763/25944.pdf |
| |
|
| |
|
|
| Regost, Christelle; Arzel, Jacqueline; Robin, Jean; Rosenlund, Grethe; Kaushik, Sadasivam. |
| The aim of the study was to investigate the replacement of fish oil by vegetable oils and the effects of a washout with a return to fish oil on growth performances and lipid metabolism. three experimental fish meal-based isonitrogenous (crude protein content: 57.5%) and isolipidic (crude lipid content: 16.5%) diets were formulated containing either 9% of added fish oil (170), soybean oil (SO), or linseed oil (LO). Each diet was distributed to triplicate groups of 25 marketable size turbot (initial body weight of 579 g) grown in seawater at water temperature of 17 T. Fish were fed once a day to visual satiety. At the end of the growth trial, which lasted 13 weeks, all groups of turbot were fed with FO diet for 8 weeks. The growth of turbot was high, but the... |
| Tipo: Text |
Palavras-chave: Lipogenesis; Fatty acids; Liver; Muscle; Body composition; Growth; Vegetable oil; Fish oil; Psetta maxima. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-375.pdf |
| |
|
| |
|
|
| Medale, Francoise; Boujard, Thierry; Vallee, Frédéric; Blanc, Denise; Mambrini, Muriel; Roem, Arjen; Kaushik, Sadasivam. |
| High energy extruded diets were formulated to contain the same level of protein supplied either by soy protein concentrate (SPC) or fish meal. Three experiments were performed in order to measure voluntary feed intake and feed waste, faecal losses and soluble losses of nitrogen and phosphorus in rainbow trout (average body weight: 100 g). Voluntary feed intake and growth performance of fish fed with demand feeders were not different when diets contained 0, 50 or 75 % SPC instead of fish meal. Total replacement of fish meal by SPC led to a significant decrease in feed intake and resulted in poor growth. This was partly due to methionine deficiency in the SPC based diet. With the addition of crystalline DL-methionine in the diets, an improvement of feed... |
| Tipo: Text |
Palavras-chave: Digestibilité; Excrétion; Azote; Phosphore; Protéine de soja; Ingestion volontaire; Truite arc-en-ciel; Digestibility; Excretion; Nitrogen; Phosphorus; Soy protein; Voluntary feed intake; Rainbow trout. |
| Ano: 1998 |
URL: https://archimer.ifremer.fr/doc/1998/publication-871.pdf |
| |
|
| |
| Registros recuperados: 40 | |
|
|
|
